Vacuolar sequestration of glutathione S-conjugates outcompetes a possible degradation of the glutathione moiety by phytochelatin synthase
Monochlorobimane was used as a model xenobiotic for Arabidopsis to directly monitor the compartmentation of glutathione-bimane conjugates in situ and to quantify degradation intermediates in vitro. Vacuolar sequestration of the conjugate was very fast and outcompeted carboxypeptidation to the γ-glut...
| Authors: | ; ; ; ; |
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| Format: | Electronic Article |
| Language: | English |
| Check availability: | HBZ Gateway |
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| WorldCat: | WorldCat |
| Fernleihe: | Fernleihe für die Fachinformationsdienste |
| Published: |
Wiley
3 November 2006
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| In: |
FEBS letters
Year: 2006, Volume: 580, Issue: 27, Pages: 6384-6390 |
| Further subjects: | B
cysteinylglycine-bimane
B PCS B cysteine-bimane B GST B Monochloro-bimane B phytochelatin synthase B MCB B γ-glutamylcysteine-bimane B glutathione S-bimane B CysGly-B B glutathione S-transferase B Glutathione-conjugate degradation B GSB B monochlorobimane B low molecular weight thiol B Pi B Cys-B B γ-EC-B B γ-glutamyl-transferase B LMWT |
| Online Access: |
Volltext (kostenfrei) Volltext (kostenfrei) |
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| 245 | 1 | 0 | |a Vacuolar sequestration of glutathione S-conjugates outcompetes a possible degradation of the glutathione moiety by phytochelatin synthase |c Anke Grzam, Pierre Tennstedt, Stephan Clemens, Rudiger Hell, Andreas J. Meyer |
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| 520 | |a Monochlorobimane was used as a model xenobiotic for Arabidopsis to directly monitor the compartmentation of glutathione-bimane conjugates in situ and to quantify degradation intermediates in vitro. Vacuolar sequestration of the conjugate was very fast and outcompeted carboxypeptidation to the γ-glutamylcysteine-bimane intermediate (γ-EC-B) by phytochelatin synthase (PCS) in the cytosol. Following vacuolar sequestration, degradation proceeded to cysteine-bimane without intermediate. Only co-infiltration of monochlorobimane with Cd2+ and Cu2+ increased γ-EC-B formation to 4% and 25%, respectively, within 60 min. The role of PCS under simultaneous heavy metal stress was confirmed by investigation of different pcs1 null-mutants. In the absence of elevated heavy metal concentrations glutathione-conjugates are therefore first sequestered to the vacuole and subsequently degraded with the initial breakdown step being rate-limiting. | ||
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| 650 | 4 | |a cysteinylglycine-bimane | |
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| 650 | 4 | |a glutathione S-bimane | |
| 650 | 4 | |a glutathione S-transferase | |
| 650 | 4 | |a GSB | |
| 650 | 4 | |a GST | |
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| 650 | 4 | |a low molecular weight thiol | |
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| 650 | 4 | |a monochlorobimane | |
| 650 | 4 | |a Monochloro-bimane | |
| 650 | 4 | |a PCS | |
| 650 | 4 | |a phytochelatin synthase | |
| 650 | 4 | |a Pi | |
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| 650 | 4 | |a γ-glutamylcysteine-bimane | |
| 650 | 4 | |a γ-glutamyl-transferase | |
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| STA | 0 | 0 | |a Pi |
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| STC | 0 | 0 | |a Pi |
| STD | 0 | 0 | |a Pi |
| STE | 0 | 0 | |a 圆周率 |
| STF | 0 | 0 | |a 圓周率 |
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